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. 2019 Sep 7;11(9):520. doi: 10.3390/toxins11090520

Figure 3.

Figure 3

Construction of hosA and hdaA complementation strains and phenotypic plates. (A) hosA and hdaA were complemented with a hygromycin selection marker and the HDACs with their own promoters and terminators. (B) Agarose gel electrophoresis of the PCRs that were used to confirm that the hosA and hdaA expression cassette were integrated into the genome. Lane M, DL5000 bp DNA Marker (Takara); lane 1, PCR-amplified DNA fragments using the primer pair of hosA shows as 4F and 4R; lane 3, PCR-amplified DNA fragments using the primer pair of hdaA shows as 4F and 4R; lane 2 and lane 4 were control groups. The template of each PCR is marked on each lane. (C) Morphological phenotypes of wild-type strains, the ΔhosA and ΔhdaA mutants and the complementation strains under culture conditions in which the phenotypes of the two mutants varied significantly.