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RT-qPCR and NGS show high correlation in determining levels of EGFR and presence of EGFRvIII. (A) EGFR expression by RT-qPCR is highly correlated with expression derived from NGS data. (B) Samples in which EGFRvIII expression is identified by RT-qPCR (ie, ΔCt < 30) often also show detectable levels of EGFRvIII by panel-based RNA-seq.
