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. 2019 Oct 9;19:150. doi: 10.1186/s12935-019-0857-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of overexpression of PIK3CD-AS1 on EMT of hepatocellular carcinoma cells. a Observation of cell morphology by an inverted microscope (×200); b detection of ZEB1 and Snail mRNA expression in cells by RT-qPCR; c detection of E-cadherin and Vimentin protein expression in cells by immunofluorescence staining (×400); d detection of E-cadherin and Vimentin protein expression by western blot analysis; the experiment was repeated three times; the data was analyzed by the t test or one-way ANOVA, and after ANOVA, the pairwise comparison was analyzed by the LSD-t test; *P < 0.05 vs the blank group