Table 2.
Detected phosphopeptides at 30 °C and 18 °C reactions, with each phosphorylated residue marked with star
| Sequence | Theo.MH+ [Da] | DeltaM [ppm] | Site1 | Site2 | Site3 |
|---|---|---|---|---|---|
| 30 °C | |||||
| FS*EHYHDAWASR | 1585.62198 | − 0.15 | 2863 | ||
| IENS*WVYGENWSDSQK | 2021.82768 | 6.01 | 2878 | ||
| KIENS*WVYGENWSDSQK | 2149.92264 | 0.14 | 2878 | ||
| KIENS*WVYGENWSDS*QKAHPR | 2611.17254 | − 1.34 | 2888 | ||
| YKEPVRES*LK | 1328.66099 | 0.88 | 2916 | ||
| EPVRES*LK | 1037.5027 | 0.5 | 2916 | ||
| ALLAIGWSVEHS*EVDIPSTNR | 2374.14387 | 2.47 | 2930 | ||
| ALLAIGWSVEHSEVDIPS*TNRSSMR | 2835.34952 | 5.99 | 2936 | ||
| RQS*KSGGRPVDVVTDSATPFNYNPHPVDMTNLTLSR | 4052.92842 | 1.35 | 2946 | ||
| S*GGRPVDVVTDSATPFNYNPHPVDMTNLTLSR | 3553.64174 | 10.58 | 2948 | ||
| SGGRPVDVVTDS*ATPFNYNPHPVDMT*NLT*LSR | 3713.5744 | 12.04 | 2959 | 2973 | 2976 |
| SGGRPVDVVTDSATPFNYNPHPVDMT*NLTLSR | 3537.64682 | 9.43 | 2973 | ||
| S*QEFLK | 831.36481 | − 0.65 | 3033 | ||
| DRERS*QEFLK | 1387.63657 | − 0.1 | 3033 | ||
| ERS*QEFLK | 1116.50852 | 0.42 | 3033 | ||
| 18 °C | |||||
| KIENS*WVYGENWSDSQK | 2149.92264 | − 0.2 | 2878 | ||
| ERS*QEFLK | 1116.50852 | 1.07 | 2916 | ||
| ALLAIGWSVEHSEVDIPS*TNRSSMRR | 3007.44554 | 1.03 | 2936 | ||
| YKEPVRES*LK | 1328.66099 | − 0.45 | 3033 | ||