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. 2019 Jun 30;9(3):84. doi: 10.3390/bios9030084

Figure 4.

Figure 4

Optimization of experimental conditions for detection of tau381. pH value (A): 6.6, 6.8, 7.0, 7.2, and 7.4; aptamer concentration; (B): 2, 3, 4, 5, and 6 μM; aptamer-tau381 incubating time; (C): 5, 10, 15, 20, 30, 45, and 60 min; dilution ratio of serum; (D): 1:50, 1:100, 1:200, and 1:500, respectively. The concentration of tau381 was 20 pM and the current signal was obtained by the DPV which was carried out in 0.1 M PBS (pH 7.0) from −0.6 to 0.2 V.