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. 2018 Sep 15;219(4):599–608. doi: 10.1093/infdis/jiy552

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Figure 4. — Increased production of interleukin 4 (IL-4) and interleukin 13 (IL-13) in splenic cells of ibrutinib-treated mice. Splenocytes were harvested on day 20 after infection, and single-cell suspensions were prepared and restimulated with Leishmania donovani freeze-thaw antigen for 72 hours. Cytokine production was analyzed in the culture supernatants by ELISA. Interleukin 4 (IL-4; A), interleukin 13 (IL-13; B), interleukin 10 (IL-10; C), interferon γ (IFN-γ; D), and tumor necrosis factor α (TNF-α; E) cytokines in antigen restimulated splenocytes of ibrutinib-treated and vehicle-treated mice. F, Levels of nitric oxide (NO) production in splenocyte culture supernatants was measured by the Greiss assay. Data are mean ± standard error of the mean and represent the values of one of the 3 independent experiments, with ≥5 mice/group. *P < .05 and **P < .01, by the unpaired t test.