Figure 6.

Ibrutinib treatment affects the immune populations important for visceral leishmaniasis exacerbation. Livers and spleens were harvested from ibrutinib-treated and vehicle-treated mice on day 28 after infection and analyzed for myeloid cell populations by flow cytometry. A, Representative dot plots of myeloid cell populations from the livers and spleens of vehicle- and ibrutinib-treated mice. P1 represents the inflammatory monocytes (CD11b⁺Ly6C^highLY6G⁻), P2 represents macrophages (CD11b⁺Ly6C^low LY6G⁻), and P3 represents neutrophils (CD11b⁺Ly6C⁻LY6G⁺). B, Frequencies of Ly6C^hi cells in the total number of CD11b gated cells in the livers and spleens of vehicle- and ibrutinib-treated mice. Data are representative of one of the 3 independent experiments, with 5 mice/group. *P < .05, by the unpaired t test.