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. Author manuscript; available in PMC: 2019 Oct 9.
Published in final edited form as: Sci Transl Med. 2019 Jul 3;11(499):eaav4634. doi: 10.1126/scitranslmed.aav4634

Fig. 4. Metabolism of P. aeruginosa isolates is consistent with adaptation to succinate.

Fig. 4.

(A) Nonsynonymous mutations (NSM) in 17 P. aeruginosa isolates (SCV and mucoid variants) compared with PAO1 control. #: stop codon mutations. (B) Fold mRNA expression relative to PAO1 for selected metabolic genes in SCV 686 and mucoid 605 (n = 3). (C) Relative single-carbon source assimilation for PAO1, SCV 686, and mucoid 605 strains (n = 6). (D) Bacterial final point growth in increasing concentrations of succinate in LB (n = 3). (E) Bacterial biofilm in increasing concentrations of succinate in LB (n = 3). (F) Relative mRNA expression against control PAO1 by quantitative reverse transcription polymerase chain reaction (qRT-PCR) of genes that regulate c-di-GMP and EPS required to produce biofilm (n = 3). (G) Regulation of metabolic genes by succinate in P. aeruginosa SCV 686 and mucoid 605 clinical isolates (n = 3). Data are shown as means ± SEM. (B and F) One-way ANOVA; (D and E) two-way ANOVA. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05.