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. 2019 Sep 25;286(1911):20191644. doi: 10.1098/rspb.2019.1644

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© 2019 The Author(s)

Published by the Royal Society. All rights reserved.

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Figure 1. — Effect of cryopreservation on sperm quality indicators: (a) motility (paired t-test: t₁₄ = 12.1, p < 0.001), (b) percentage of fast progressive sperms (t₁₄ = 9.7, p < 0.001) and (c) the average path velocity VAP (t₁₄ = 2.3, p = 0.04). (d) The fertilization success of the 40 males used in the breeding experiment, based on 2 × 12 eggs per male (see table 1a for statistics). Plots show means and 95% confidence intervals for non-cryopreserved sperm (empty bars or symbol) and cryopreserved sperm (filled bars or symbol). Asterisks indicate the levels of significance (***p < 0.001; *p < 0.05; ‘n.s.’, not significant).