Table 2.

Treatment effects on larval traits. LRTs comparing linear mixed-effects models on (a) length at hatching, (b) yolk sac volume at hatching and (c) larval growth. Models including or lacking the term of interest were compared with the reference model in italics. Significant p-values are highlighted with bold type. Fixed effects: p, pathogen treatment; c, cryopreservation; l, length at hatching; random effects: m, male; b, breeding block.

model terms	effect tested	AIC	d.f.	χ2	p-value
(a) length at hatching
p + c + m + b		821	6
p + c + m	b	851	5	32.1	<0.001
p + c + b	m	828	5	9.4	0.002
p+ m + b	c	819	5	0.2	0.61
c + m + b	p	866	5	47.0	<0.001
p + c + m + c|b	c × b	820	8	4.8	0.09
p + c + m + p|b	p × b	823	8	2.2	0.34
p + c + c|m + b	c × m	824	8	0.5	0.79
p + c + p|m + b	p × m	832	8	0	1.0
p + c + p|c + m + b	p × c	821	7	2.2	0.14
(b) yolk volume at hatching
p + c + m + b		4273	6
p + c + m	b	4362	5	90.8	<0.001
p + c + b	m	4271	5	0	1.0
p + m + b	c	4272	5	0.8	0.36
c + m + b	p	4272	5	1.0	0.33
p + c + m + c|b	c × b	4273	8	4.0	0.13
p + c + m + p|b	p × b	4276	8	1.4	0.49
p + c + c|m + b	c × m	4275	8	2.0	0.37
p + c + p|m + b	p × m	4277	8	0.2	0.91
p + c + p|c + m + b	p × c	4275	7	0.1	0.72
(c) larval growth
l + p + c + m + b		488	7
l + p + c + m	b	492	6	6.0	0.01
l + p + c + b	m	488	6	2.4	0.12
l + p + m + b	c	491	6	5.2	0.02
l + c + m + b	p	489	6	3.5	0.06
p + c + m + b	l	486	6	0.3	0.56
l + p + c + m + c|b	c × b	492	9	0.2	0.91
l + p + c + m + p|b	p × b	491	9	1.2	0.54
l + p + c + m + l|b	l × b	490	9	1.6	0.46
l + p + c + c|m + b	c × m	492	9	0.4	0.83
l + p + c + p|m + b	p × m	492	9	0.1	0.95
l + p + c + l|m + b	l × m	483	9	8.5	0.01
l + p + c + p|c + m + b	p × c	490	8	<0.1	0.84
l + p + c + l|c + m + b	l × c	488	8	1.5	0.23
l + p + l|p + c + m + b	l × p	490	8	<0.1	0.99