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. 2019 Jun 10;4(9):1465–1474. doi: 10.1038/s41564-019-0475-9

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© The Author(s), under exclusive licence to Springer Nature Limited 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — All panels are the results from a single experiment. a−c, 3D volume rendering of reconstructed ‘G. incantans’, the rER and the nucleus of a fibre cell, superimposed on a virtual slice of the 3D TEM tomography stack; the rER has been virtually removed to partially (b) and fully (c) show the symbionts within the rER lumen. No scale bar is shown as the scale varies with perspective. d−i, Selected tomography slices (on which the 3D reconstruction is based) at various depths (see z values indicated) show the connection between the nucleus, the rER and the bacteria. For ease of interpretation, d has been false-coloured using the colour key from a. For raw data, see Supplementary Fig. 8.