Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 15;44(8):1377–1388. doi: 10.1038/s41386-018-0274-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — FAAH overexpression in hippocampal CA1-CA3 pyramidal neurons leads to decreased anandamide (AEA) and palmitoylethanolamide (PEA) levels. a AEA and PEA levels were significantly lower in AAV-Glu-FAAH samples (n = 11-12) as compared to control AAV-WT animals (n = 15-16) and AAV-empty injected mice (n = 12-14), whereas oleoylethanolamide (OEA), arachidonic acid (AA), and 2-arachidonoylglycerol (2-AG) levels were not altered. b FAAH activity is enhanced upon FAAH overexpression in hippocampus. Along increasing amounts of substrate [³H]-AEA (0–50 μM range), FAAH activity was increased in AAV-Glu-FAAH (n = 3) as compared to AAV-WT (n = 3) mice as indicated by the increase in the maximal amount of product [³H-ethanolamine], and the statistically significant increase in V_max (inset). No significant change in K_m constant was found. Data are expressed as amount of product [³H-ethanolamine] (pmol) generated over reaction time (min) and protein amount (mg). *p < 0.05, **p < 0.01, ***p < 0.001 one-way ANOVA with Tukey’s multiple comparison test. V_max and K_m values were obtained by using a Michaelis-Menten equation-based analysis. Values are expressed as means ± SEM. Statistical analysis details are reported in Supplementary Table S3.