Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 15;44(8):1377–1388. doi: 10.1038/s41386-018-0274-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Long-term plasticity at hippocampal CA3-CA1 synapses. a Schematic drawing illustrates placement of stimulation and recording electrodes in the hippocampal slice. b Discharge pattern of CA1 neurons upon depolarizing (500 pA) and hyperpolarizing (−250 pA) current pulses. Scale bars: 50 mV, 200 ms. c Number of generated action potentials (APs) plotted against positive current injection (in pA) in CA1 pyramidal cells of AAV-WT and AAV-Glu-FAAH mice. d, e LTP in CA1 following high-frequency stimulation (HFS, 100 Hz) is significantly enhanced in AAV-Glu-FAAH mice compared to AAV-WT mice (unpaired t test, *p < 0.05). d Inset shows sample EPSC traces at indicated time points before and after HFS, scale bars: 100 pA, 25 ms. f PPI does not change after induction of LTP. g Analysis of the coefficient of variation (CV²) plotted against mean EPSC ratios indicates a presynaptic mechanism involved in LTP in AAV-Glu-FAAH mice and a postsynaptic one in AAV-WT mice. h, i Excitatory LTD (eLTD) at CA3-CA1 synapses following low-frequency stimulation (LFS, 900 paired pulses, 1 Hz) is unchanged in AAV-Glu-FAAH mice compared to AAV-WT mice. h Inset shows sample EPSC traces at indicated time points before and after LFS, scale bars: 100 pA, 25 ms. j PPI does not change after induction of eLTD. k Analysis of CV² plotted against mean EPSC ratios point to a postynaptic mechanism involved in eLTD in both genotypes. l, m. Both AAV-WT and AAV-Glu-FAAH mice showed intact inhibitory LTD (iLTD) at CA3-CA1 synapses. In (l), inset shows sample IPSC traces at indicated time points before and after HFS, scale bars: 200 pA, 25 ms. n PPI does not change after induction of iLTD. o Analysis of CV² plotted against mean IPSC ratios indicates a presynaptic mechanism involved in iLTD in mice of both genotypes. In e, i, m, scatter plots and corresponding mean ± SEM represent amplitudes recorded after plasticity induction (LTP and iLTD: min 36–40; eLTD: min 46–50). In c, d, h, l, numbers indicate the number of recorded cells/animals.