Fig. 2.

At higher magnification, variability in the Golgi complex staining pattern can be observed. Fibers from soleus muscles were double-stained with MG160 (mg) and GLUT4 (gt) and observed with a conventional fluorescence microscope. Top panel shows the MG160 staining at the surface of a fiber. Notice that some nuclei only show staining in the equatorial plane, whereas others (arrows) show staining above and below as well. In thebottom panel, also focused on the surface of a fiber, we observe three nuclei, visualized by Hoechst staining (h). Two of them (arrowheads) show perinuclear staining for both MG160 and GLUT4. The third nucleus (arrow), has a very localized and strong MG160 labeling, but no GLUT4 labeling, which identifies it as a nonmuscle nucleus, associated with, but outside of the muscle fiber. Scale bars, 10 μm.