Fig. 5.

Single-channel recording from membrane patches expressing heat-sensitive ion channels in cell-attached patch and inside-out patch configuration. Bath solution in these experiments contained 130 mm K⁺, and the pipette solution contained 130 mm Na⁺. The pipette potential was set to +60 mV. A, Heat ramp from 37°C to 51°C evokes multiple channel activity in cell-attached patch configuration (n = 19 of 97). The activity starts at 44°C and stops when the temperature returns to 37°C.B, Sweeps (100 msec) of a recording from a heat-sensitive membrane patch (cell-attached configuration) showing channel activity (upward deflections) at 51°C. C, SCG neuron membrane patch in cell-attached configuration, showing no heat-activated channel activity. D, Temperature dependence of channel activity in a cell-attached membrane patch stimulated by a slow heat ramp. The dotted line shows shut level. E, Mean single-channel current (▪, mean ± SEM of 8 cell-attached patches) as a function of temperature, compared with the mean whole-cell current (○, mean of 23 cells). F, Heat-induced channel activity at 50°C in the same membrane patch, recorded in cell-attached and inside-out mode.