Table 2.

RT-PCR transcript levels in glial scar, uninjured cortex, and cultured astrocytes

	GFAP mRNA	Phosphacan mRNA	Neurocan mRNA
Glial scar implant	≫50 fg	0.3 fg	2.5 fg
Uninjured cortex	25 fg	0.1 fg	0.3 fg
Cultured astrocytes	20 fg	<0.1 fg	3.5 fg

RT-PCR amplification from cDNA pools normalized to equivalent levels of GAPDH expression. The cDNA amplified for GFAP was produced from the equivalent of 5 ng of total RNA. For phosphacan and neurocan, cDNA from the equivalent of 15 ng of total RNA was used in the PCR reaction.