Fig. 1.

Outline of the study protocol. Three cohorts of rats acquired from the maternal separation protocol were used for the behavioural and immunohistological studies. The above schematic outlines the time points of each experimental procedure. PND: postnatal day, MS180: Maternal separation 180 min/day procedure, NS non-separated control animals, ABT affective bias test, SPT sucrose preference test, CORT corticosterone, JBT judgement bias task, PR progressive ratio, NFST novelty suppressed feeding test