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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Immunol. 2019 Jun 12;203(3):718–724. doi: 10.4049/jimmunol.1900271

Fig 3. TRAILshort Knockout Increases TRAIL Sensitivity and T Cell Apoptosis Following Acute HIV Infection in vitro.

Fig 3.

(A) Strategy for CRISPR interruption of Exon 2 of TRAIL (TNFSF10). (B) TRAILshort expression in response to stimulation with phorbol 12-myristate 13-acetate (PMA) was assessed by western blot in parent Jurkat cells (Jurkat-wt) or two TRAILshort-KO clones (B20 and I14). (C) Surface TRAILshort expression in similarly treated cells was assessed by flow cytometry. (D) Jurkat-wt and Jurkat-I14 cells were treated with vehicle control or skTRAIL (1 ng/ml) and assessed for active Caspase 3/7 expression over time by live cell imaging. (E) Jurkat-wt and Jurkat-I14 cells were mock-infected or infected with HIV-IIIB, and and assessed for active Caspase 3/7 expression over time by live cell imaging. Data represents mean + SD for 5–6 replicates from one experiment, representative of at least three independent experiments. P values were determined by linear regression.