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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Nat Metab. 2019 Sep 16;1(9):861–867. doi: 10.1038/s42255-019-0108-x

Figure. 1: Withdrawal of exogenous serine limits proliferation and is exacerbated by inhibition of mitochondrial respiration.

Figure. 1:

a, Western blot analysis to assess expression of serine synthesis enzymes in A549 and MDA-MB-468 cells, and in MDA-MB-231 cells with (pLHCX PHGDH) and without (pLHCX EV) PHGDH expression as indicated. These experiments were repeated 3 times with similar results. b, Proliferation rates of the indicated cancer cells cultured in media with serine (+ Serine), or without serine (- Serine). c, Proliferation rates of A549 and MDA-MB-468 cells, and of MDA-MB-231 cells with (pLHCX PHGDH) and without (pLHCX EV) PHGDH expression at the indicated concentration of rotenone, in the presence or absence of exogenous serine as indicated. Data shown are mean (+/− standard deviation) of 3 biological replicates.