Fig 6. RNA polymerase I is not translocated upon TBEV infection.
(A) DAOY cells were infected with TBEV Hypr strain (MOI 5) and at indicated time intervals fixed and POLR1A was detected using rabbit anti-POLR1A and anti-rabbit DyLight594 antibodies. Cells were further co-stained for viral NS3 protein using chicken anti-NS3 and anti-chicken DyLight488 antibodies. Nuclei were stained with DAPI. Scale bar represents 200 μm. (B) Zoomed images from panel (A) at 48 hours p.i. (areas marked by the white squares); POLR1 is localised in distinct foci in host nuclei without any observable virus-induced translocation. Scale bar represents 100 μm. (C) DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of POLR1A mRNA using Δ-ct method with normalisation to the cell number was performed. Data are representative of three independent experiments and values are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (* P<0.05; ** P<0.01; **** P<0,0001).