Fig. 1.

Ethanol-induced Pavlovian learning in male DBA2/J mice. a Experimental timeline for ethanol-induced CPP paradigm. b, c No difference in preference for CS + side was observed between mice assigned to the saline group (n = 24) and mice assigned to the ethanol group (n = 24). There was also no difference in mean velocity between the two groups. d, e Following four days of conditioning with two 5 min trials per day, mice injected with ethanol showed a significant preference for the CS + side on a drug-free test day when compared to saline-treated mice. Post conditioning, mice in the ethanol-paired group showed a significant reduction in mean velocity compared to saline-paired mice. f Experimental timeline for ethanol-induced CPA paradigm. g, h) Average pretest data showing mice assigned to the saline group (n = 14) did not differ in their preference for CS + side when compared to ethanol group (n = 16). No difference in mean velocity between the two groups was observed. i, j Mice injected with ethanol immediately after 5 min exposure to a distinct cue (grid or hole floor) expressed a significant aversion for CS + side on Test day and also showed a significant reduction in mean velocity compared to saline-paired mice following ethanol-induced aversion. Data expressed as Mean ± SEM.*p < 0.05