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Schematic representation of the decoy generation approach used by GDG. Decoys are generated via two main steps: First, a glycan is randomly selected from a pool of about 300 biologically relevant N-linked glycans separated in three categories (see text); and second, an arbitrary mass, representing the decoy’s peptide portion, is added to the glycan so that the total mass of the decoy is within a user-specified mass tolerance from the target glycopeptide mass.
