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. 2019 Sep 30;8:e48750. doi: 10.7554/eLife.48750

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© 2019, Dong et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — TIRF images of live cultured rat hippocampal neurons transfected with GFP (shown in A), or nAb-GFP fusions against Homer1 (clone HC12, shown in B), IRSp53 (clone IC65, shown in C), SAPAP2 (clone SS80, shown in D), Gephyrin (clone GC52, shown in E), or AMIGO-1 (clone AC50, shown in F). For each, two images (of the same field of view) taken one min apart are shown. To the right is an overlay of the initial image (in green) and the subsequent image (in magenta). Overlap of green and magenta yields a white signal. Arrows point to punctate structures. The column to the far right shows an analysis of the extent of overlap of pixels between the initial and subsequent images. The scale bar in the top left panel is 5 μm and holds for all panels in figure.