Table 1. Summary of nanobody generation and validation.

	Primary selection and validation			Validation as intrabodies		Validation as immunolabels
Target	Phage clones selected	ELISA positives	Unique ELISA positives	COS-1 intrabody positives	Neuron intrabody positives	COS-1 IF-ICC positives	Brain IHC positives	Brain IB positives
Homer1	180	135	39	32	12	33	25	13
IRSp53	160	33	17	8	3	0	0	0
SAPAP2	172	32	15	7	2	0	4	0
Gephyrin	182	78	24	9	5	0	1	2
AMIGO-1	173	38	18	13	5	0	0	0