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. Author manuscript; available in PMC: 2019 Oct 9.
Published in final edited form as: J Vis Exp. 2018 Dec 5;(142):10.3791/58814. doi: 10.3791/58814

Figure 4. Exosome purification post-electroporation.

Figure 4

(A) Elution profiles (F0-F9) of Atto655-siRNA and electroporated exosomes using size exclusion chormatography. (B) NTA analysis of both Atto655-siRNA and exosome from F0 to F9 using size exclusion chormatography. (C) The calibration curve of Atto655 labelled siRNA. Fluorescence intensities were obtained by plate reader at Ex/Em: 640-10/680 nm; Gain 2800. Values are expressed as mean ± SD (n = 3).