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. Author manuscript; available in PMC: 2019 Oct 9.
Published in final edited form as: J Vis Exp. 2018 Dec 5;(142):10.3791/58814. doi: 10.3791/58814

Table 1. Physicochemical characterization of exosomes.

Exosome Size1,2
(nm)		 Yield1,2,3
(p/mL)		 [Protein]2,4
(µg/mL)		 Particle-to-protein (P:P) ratio5
(p/µg)
HEK-293 107.0 ± 8.2 6.99 ± 0.22 x 1012 84.3 ± 9.8 8.3 ± 1.7 x 1010
1

Measured using nanoparticle tracking analysis (NTA instrument)

2

Values are expressed as mean ± SD, where n=3

3

Yield was obtained by cell-conditioned medium pooled from 2 rounds of harvesting from bioreactor flasks (~24 mL)

4

Measured using a protein assay kit

5

Value obtained by using formula: P:P ratio = Yield / [Protein]