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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Transfusion. 2019 Aug 12;59(10):3243–3252. doi: 10.1111/trf.15482

Figure 3. Infection-induced alloimmune responses to transfused RBCs are Type I IFN dependent.

Figure 3.

(A)Wild type C57BL/6 mice, mice lacking type 1 IFN receptors (IFNAR KO mice), or mice lacking the interferon regulatory factor (IFR) 3 and 7 pathways (IRF 3/7 double KO mice) were intranasally infected with 10 PFU of PR8 influenza virus and transfused 3 days later with K1 RBCs; anti-KEL glycoprotein IgG responses were measured by flow cytometric crossmatch. (B) 28 days after the initial K1 RBC transfusion, wild type or IFNAR KO mice were re-transfused with labeled RBCs and K1 RBC post-transfusion recovery and survival was evaluated. Data shown are representative of 2–3 independent experiments, * p<0.05; error bars depict standard deviation.