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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Anaerobe. 2019 May 8;59:1–7. doi: 10.1016/j.anaerobe.2019.05.002

Figure 2. SinR’ interacts with SinR through its Multimerization Domain.

Figure 2.

(A) In vitro, protein-protein interactions indicate that SinR’-MD binds tightly to SinR. E. coli whole cell lysates expressing various GST-tagged SinR’ proteins were incubated with SinR-6His proteins and were purified using Ni++ agarose affinity columns. The elutes were probed with Rabbit Anti-GST and with Mouse Anti-His antibodies. Lanes details are as follows:

I. Input 1: Mixture of SinR’-GST expressing E. coli lysate with purified SinR-6His

UB. Unbound from input 1 after passing through Ni++ column

W. First wash

E1. Elute with 50 mM imidazole (GST+SinR-6His)

E2. Elute with 200 mM imidazole (SinR’-GST + SinR-6His)