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. 2019 Oct 9;9:14482. doi: 10.1038/s41598-019-50848-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EVs shedding by melanoma cells after TMZ treatment do not modulate cell sensitivity to chemotherapy in vitro. In (a) EVs uptake by SKMel 37 (i,ii,iii) and B16F10 (iv,v,vi) melanoma cells. Scale bars, 50 and 40 µm. In (b) SKMel 37 and (c) B16F10 cells were treated with TMZ in the presence of EVs from pre-treated cells (DMSO or TMZ) and cell death was evaluated by flow cytometry after propidium iodide staining (upper graphs). Clonogenic assay was also performed for both cell lines (lower graphs) (n = 6–9). Bars indicate mean ± SD.