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. 2019 Oct 9;8(10):59. doi: 10.1038/s41389-019-0165-8

Fig. 1. STAT3 promotes cell proliferation, invasion, and migration.

Fig. 1

a Detection of pSTAT3 Tyr705, pSTAT3 Ser727 expression by western blot in A2780, OVCA429, HEY, and SKOV3 cells. b Detection of HA-tag, STAT3, pSTAT3 Tyr705, and pSTAT3 Ser727 in constructed OVCA429 and SKOV3 cell lines. c, d Detection of cell proliferation by MTT at the indicated time points (P < 0.05). e, f Colony-forming capability of cancer cells at 14 days. g Quantitative analysis of migrated cells. h Detection of cell invasion by using a high throughput screening multi-well insert 24-well two-chamber plates. i, j Detection of migration speed by scratch assay. k Quantitative analysis of migration speed by migration index. l Detection of metastasis-associated proteins by western blot. β-actin was used as a loading control. *p < 0.05, **p < 0.01. Error bars = 95% CIs