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. 2019 Oct 9;10:4594. doi: 10.1038/s41467-019-12568-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Segmental pattern of the vertebral lymphatic vasculature in the thoracic spine. a Alcian blue/Alizarin red staining of the mouse vertebral column with boxes indicating position of images shown in Figs. 1–4 (thoracic vertebrae) and 5 (cervical and lumbar vertebrae), spatial orientation (A: anterior, P: posterior, L: lateral, V: ventral). b Alizarin red staining of two successive thoracic vertebrae (delimited by red/blue dots, lateral view). LF: ligamentum flavum, red asterisk: ventral vertebral body, blue arrow: facet joint (FJ), red arrowhead: ventral intervertebral disk, blue asterisk: intervertebral foramen. c latero-frontal schematic drawing corresponding to (b). DM: dura mater, LM: leptomeninges (pia mater and arachnoid), SC: spinal cord, SN: spinal nerve. d Dorsal view of LYVE1 staining. Red and blue areas correspond to two successive vertebrae. Note LVs lining ligamentum flavum. e, f Dorsal (e) and lateral (f) views of the PROX1 expression pattern. Red and blue areas correspond to two successive vertebrae. Salmon arrows: intervertebral LVs, blue arrow: dorsal LVs. g, h Segmented images of the PROX1 LV network (fronto-dorsal (g) and lateral (h) views) highlighting three successive vertebral LV units (red, blue, green). Scale bars: 2 mm (a); 300 µm (b, e, f); 200 µm (d, g, h)