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. 2019 Oct 9;9(11):390. doi: 10.1007/s13205-019-1922-2

Fig. 4.

Fig. 4

Comparison of quantitative real-time reverse transcription-PCR (qPCR) analyses of selected genes (left y-axis, histograms) and fragments per kilobase of exon model per million mapped reads (FPKM) values as assessed by RNA-seq (right y-axis, dotted lines). The cucumber β-actin gene (GenBank AB010922) was used as an internal control to normalize the expression data. Each value denotes the mean relative level of expression of three biological replicates