Figure 5.

The calcium signaling pathway is overrepresented in the specific differentially expressed genes altered by alfalfa intervention during the preweaning period. (A) Molecules involved in the increase and removal of the intracellular calcium ([Ca²⁺]_i). Cav1.3, L-type Ca²⁺ channel subunit alpha 1 D; Cav1.2, L-type Ca²⁺ channel subunit alpha 1 C; STAC, SH3 and cysteine-rich domain-containing protein; RyR, ryanodine receptor; HRC, histidine-rich calcium-binding protein; FAM20C, FAM20C Golgi associated secretory pathway kinase; KCHIP2, potassium voltage-gated channel-interacting protein 2; Kv4, voltage-gated potassium channel subunit Kv4; DPLP, dipeptidyl peptidase–like protein. The arrow indicates the Ca²⁺ flux. (B) Gene network regulated by the increased [Ca²⁺]_i. Red represents the upregulated genes or the proteins encoded by the upregulated genes; blue represents the downregulated genes or the proteins encoded by the downregulated genes.