Fig. 1.

a Timeline of experiments. Rats were exposed to chronic adolescent stress (CAS) from postnatal day (PND) 38–49 and assessed during adulthood. Separate groups of rats were used for DNA methylation experiments and RNA sequencing, DNMT PCR, and corticosterone experiments. b Plasma corticosterone was assessed at baseline and following acute stressor exposure (30 min following acute swim stress). A three-way analysis of variance (ANOVA) (sex × adolescent stress × acute stress) revealed a significant main effect of acute stress (*), adolescent stress (#), and sex (&). Furthermore, there was a significant sex by acute stress interaction and a significant adolescent by acute stress interaction (p < 0.05). Data are represented as mean + SEM