Table 1. Modeling parameters for simulation.

	Interpunctum interval (μm)
Parameter	1.4	2	2.5	3	3.5	4
τ (ms)	2.96	2.62	2.33	2.28	1.99	2.00
g0	9.17	8.85	8.77	8.70	9.35	9.71
g1	−3,058	−2,950	−4,386	−4,348	−4,673	−4,854
1/rcs (ms)	22.0	22.4	31.7	17.6	35.7	39.7
b	−0.869	−0.852	−0.742	−0.814	−0.694	−0.677
1/rso (ms)	470	350	317	258	397	463
d	−0.194	−0.247	−0.262	−0.332	−0.304	−0.31
α	0.458	0.466	0.459	0.482	0.511	0.52
is/io	0.216	0.287	0.348	0.32	0.469	0.527
SED (kPa)	7.69	4.22	2.99	2.19	1.34	0.87

Parameters were derived from fitting the model to the dataset from Eastwood et al. (2015), as described in Sanzeni et al. (2019). We show parameter sets derived from fitting based on the 1.4-µm IPI used in the majority of the article, as well as with longer IPIs. τ represents the time constant of relaxation for the linker, while g₀ and g₁ are dimensionless parameters relating linker elongation to the free energy of the channel. The reaction rate for transitioning from C $\rightleftharpoons$ S is controlled by a base rate r_cs and a factor b that controls how dependent the final rate is on the free energy change (Sanzeni et al., 2019, Appendix 12). Similarly, r_so and d control the reaction rate for the S $\rightleftharpoons$ O transition. α is the ratio of the free energy difference between C and S over the free energy difference between C and O. By combining these parameters with the recording conditions, we can calculate the ratio of the single-channel current in the subconductance state (i_s) over the current carried by a fully open channel (i_o). The SED is not a fitted parameter: rather, it was derived from single-channel dynamics as discussed in Materials and methods and represents the energy available to the channel as a result of deformation due to step stimulation.