Figure 4. Bcl-2, Bax and caspase-3 mRNA level alterations in control and treated cells. a: Bcl-2 mRNA level decreases after treatment and the most reduction was observed in combination drug (VPA+NG) treatment. Bax and caspase-3 mRNA levels increase after treatment. Lane M: DNA marker, Lane 1: untreated cells, Lane 2: cells treated with Nitroglycerine (NG). Lane 3: cells treated with Valproic acid (VPA). Lane 4: cells treated with combination drug (NG+VPA). b: Charts of Bcl-2, Bax and caspase-3 mRNA alterations after treatment. Basis of electrophoresis results the chare have been designed. The most reduction in Bcl-2 mRNA level occurs in combination drug treatment, and the most elevation in Bax mRNA level occurs in combination drug treatment, the most caspase-3 mRNA level elevation was observed on valproic acid treatment. Different letters indicate significant differences between groups (p<0.05).