Table 1. Summary of Binding Energies for the Systems Tested Here^a.

system	site	lipid	membrane	PMF	FEP/ABFE	WTMetaD
Kir2.2	3	PIP2	POPC	–45 ± 2	–48 ± 2	–45 ± 6
AAC	1	CDL	POPC	–7 ± 2	–10 ± 4	–14 ± 3
	2			–8 ± 4	–11 ± 2	–13 ± 3
	3			–7 ± 4	–9 ± 4	–11 ± 6
LeuT	1	CDL	POPE	–6 ± 3	–9 ± 2	–7 ± 3
A2AR	1	chol	POPC		–5 ± 1	–8 ± 3
	2				–8 ± 1	–9 ± 3
	3				–2 ± 1	–5 ± 2

^aReported are the measured ΔΔG_bind values, with the errors calculated as defined in the text and figure legends. These data have been used to populate the chart in Figure 7.