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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Cell Stem Cell. 2019 Aug 1;25(2):273–289.e5. doi: 10.1016/j.stem.2019.07.007

Figure 3: Ablation of HNF1A drives pancreatic endocrine differentiation toward cells with a more alpha cell gene expression signature.

Figure 3:

Mel1 HNF1A WT, HET and KO ESCs were differentiated into pancreatic endocrine cells and analyzed as described below.

(A) Flow cytometry of C-Peptide versus GLUCAGON at the end stage differentiation cultures (n=6 per sample).

(B–D) Quantification of pancreatic hormone expressing cells from A (n=6 per sample).

(B) C-Peptide+ (C-PEP+) monohormonal cells. (C) GLUCAGON+ (GCG+) monohormonal cells. (D) C-Peptide+ GLUCAGON+ (C-PEP+GCG+) cell population.

(E–F) mRNA time-course analysis during pancreatic differentiation at DE, PP1, PP2 and beta-like cell stages (n=3 per sample). (E) PAX4 mRNA expression. (F) ARX mRNA expression.

(G) Relative gene expression of alpha cell specific genes at end stage of differentiation in HNF1A allelic series (n=3 per sample).

For all statistical analyses: * P<0.05. See also Figure S3.