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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Cell Stem Cell. 2019 Aug 1;25(2):273–289.e5. doi: 10.1016/j.stem.2019.07.007

Figure 5: Disruption of HNF1A leads to an abnormal expression of genes related with beta cell function, development and diabetes.

Figure 5:

Mel1 HNF1A WT, HET and KO ESCs were differentiated into pancreatic endocrine cells and analyzed as described below.

(A–B) HNF1A allelic series genome profiling using purified INS-GFP+ NKX6.1+ cells at end stage of differentiation (n=3 per sample). (A) Flow cytometry plot for sorting double positive INS-GFP+ NKX6.1+ cells. (B) 501 differential expressed genes presented in a heat map plot obtained using a microarray analysis from samples sorted in A.

(C) Differential expressed genes shown in the following categories: top downregulated genes, top upregulated genes, cellular respiration and genes differentially expressed in HET compared to KO.

(D–G) qRT-PCR gene expression validation of a subset of gene targets in B (n=3 per sample). (D) Mouse HNF1A target genes. (E) Pancreas transcription factors. (F) cell stress and insulin biosynthesis. (G) Cellular respiration.

For all statistical analyses: * P<0.05, **P<0.01. See also Figure S5.