Mel1 HNF1A WT, HET and KO ESCs were differentiated into pancreatic endocrine cells and analyzed as described below.
(A–B) HNF1A allelic series genome profiling using purified INS-GFP+ NKX6.1+ cells at end stage of differentiation (n=3 per sample). (A) Flow cytometry plot for sorting double positive INS-GFP+ NKX6.1+ cells. (B) 501 differential expressed genes presented in a heat map plot obtained using a microarray analysis from samples sorted in A.
(C) Differential expressed genes shown in the following categories: top downregulated genes, top upregulated genes, cellular respiration and genes differentially expressed in HET compared to KO.
(D–G) qRT-PCR gene expression validation of a subset of gene targets in B (n=3 per sample). (D) Mouse HNF1A target genes. (E) Pancreas transcription factors. (F) cell stress and insulin biosynthesis. (G) Cellular respiration.
For all statistical analyses: * P<0.05, **P<0.01. See also Figure S5.