KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-HNF1A | Cell Signaling | Cat# D7Z2Q |
| Biotinylated goat polyclonal anti PDX-1/IPF1 | R&D | Cat# BAF2419 |
| Mouse IgG1 monoclonal anti-NKX6.1 | DSHB | Cat# F55A10 |
| Rat monoclonal anti-Somatostatin | Santa Cruz | Cat# sc-47706 |
| Mouse IgG1 monoclonal anti-Glucagon | Sigma | Cat# G2654-.2ML |
| Mouse IgG1 monoclonal anti-INSULIN | Sigma | Cat# I2018-.2ML |
| Rabbit monoclonal anti-C-Peptide | Cell Signaling | Cat# 4593S |
| Goat anti mouse IgG1–488 | Jackson Immunoresearch | Cat# 115–545-205 |
| Goat anti mouse IgG1-PE | Jackson Immunoresearch | Cat# 115–115-205 |
| Goat anti mouse IgG1–647 | Jackson Immunoresearch | Cat# 115–605-205 |
| Goat anti rabbit alexa 647 | Invitrogen | Cat# A21245 |
| Goat anti rabbit IgG-PE | Jackson Immunoresearch | Cat# 111–116-144 |
| Goat anti Rat alexa flu 647 | Thermo Fischer scientific | Cat# A21247 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Activin A | R&D | Cat#338-AC |
| Chir99021 | Cayman | Cat# 13122 |
| BFGF | R&D | Cat# 233-FB |
| FGF7 | R&D | Cat# 251-KG-050/CF |
| SANT-1 | Sigma-Aldrich | Cat# S4572 |
| Retinoic Acid | Sigma-Aldrich | Cat# R2625 |
| LDN-193189 | STEMGENT | Cat# 04–0074 |
| Phorbol 12-myristate 13-acetate | Tocris | Cat# 1201 |
| Heparin | Sigma | Cat# H3149 |
| Zinc Sulfate | Sigma | Cat# 1724769 |
| T3 (250 mg) | Sigma | Cat# T6397 |
| ALK5 inhibitor | Enzo Life Sciences | Cat# ALX-270–445 |
| GSIXX | Calbiochem | 565789 |
| N-Acetyl-L-cysteine | Sigma | A9165 |
| Trolox | EMD | Cat#648471 |
| R428 | SelleckChem | S2841 |
| Critical Commercial Assays | ||
| CisBio insulin test | Cisbio | 62INSPEB |
| XF cell mito Stress test kit | Agilent Technologies | 103015–100 |
| XF glycolysis stress test kit | Agilent Technologies | 103020–100 |
| Deposited Data | ||
| Microarray data set | This paper | https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-8120 |
| Experimental Models: Cell Lines | ||
| hESC-Mel1 INS-GFP | Dr. Stanley EG | |
| hESC-Mel1 INS-GFP- HNF1A-HET-C22 | This paper | |
| hESC-Mel1 INS-GFP-HNF1A-KO-C5 | This paper | |
| hESC-Mel1 INS-GFP-LINKA-DEL-C211 | This paper | |
| hESC-H1 | Dr. Thomson J | |
| hESC-H1-HNF1A-HET-C3 | This paper | |
| hESC-H1-HNF1A-KO-C21 | This paper | |
| hESC-H1-HNF1A-LINKA-DEL-C20 | This paper | |
| Oligonucleotides | ||
| Primers for quantitative RT-PCR | This paper | Table S1 |
| Recombinant DNA | ||
| pCas9_GFP | Addgene | 44719 |
| gRNA empty vector | Addgene | 41824 |
| Software and Algorithms | ||
| FlowJo | Ashland | https://www.flowjo.com/solutions/flowjo/downloads |
| Wave Desktop | Agilent | https://www.agilent.com/en/products/cell-analysis/software-download-for-wave-desktop |
| GraphPad Prism 6 | GraphPad Software | https://www.graphpad.com/support/faqid/%201952 |
| XLSTAT | Addinsoft | https://www.xlstat.com/en/solutions/base |
| Illustrator | Adobe System Software | https://www.adobe.com/cn/products/cs6/illustrator.html |
| ApE plasmid Editor | M. Wayne Davis | http://biologylabs.utah.edu/jorgensen/wayned/ape/ |
| Leica Application Suite X | Leica | https://www.leicamicrosystems.com/products/microssoftware/details/product/leica- |
| Illustrator | Adobe System Software | https://www.adobe.com/cn/products/cs6/illustrator.html |