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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Pept Sci. 2019 May 9;25(6):e3173. doi: 10.1002/psc.3173

Figure 3: GPX-like activity and stability of Sec- and (αMe)Sec-containing peptides.

Figure 3:

(A) GPX-like activity of peptides 11 (blue) and 12 (red) assayed with H2O2. GSSG is formed as a by-product of the peptide catalytic cycle (Figure 1). GR reduces GSSG back to GSH using NADPH, and the consumption of NADPH can be measured via absorbance at 340 nm. (B) Percent activity remaining after incubation of 11 and 12 in 50 mM potassium phosphate buffer, pH 8.0, after 24 h and 96 h.