Figure 2.

Depletion of autophagy-related proteins reduced BEFV production. MDBK or BHK-21 cells were transfected with autophagy-related gene shRNAs including A Beclin 1, B Atg7, C LC3, and D LAMP2, respectively for 24 h followed by infection with BEFV at a MOI of 1 and collected at 24 hpi. The expression level of the BEFV M protein in cell lysates was determined by Western blot and the culture medium was used for virus titration assays. All data shown represent the mean ± SD calculated from three independent experiments. Signals were quantified using Image J software. The levels of indicated proteins in the mock controls (un-infection or un-treatment) were considered onefold. The protein levels were normalised to those for β-actin. The activation and inactivation folds indicated below each lane were normalized against values for the mock control. The predicted size of each protein was labeled at the right-hand side in kDa.