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. 2019 Oct 10;17:339. doi: 10.1186/s12967-019-2087-9

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The study methodology overview and verification. a Cell-free DNA (cfDNA) was extracted from blood samples which were collected from 32 lung cancer patients during each chemotherapy cycle. Square with light scarlet referred to the first-line treatment: platinum-based chemotherapy. b Gene collections of Oseq-ctDNA panel which involves 508 cancer-related genes. c Bioinformatic pipeline for candidate mutations detecting. d Landscape of genetic alterations between 27 pre-chemotherapy samples and TCGA cohort. A bar represented one of six substitution subtypes with different colors. The y-axis represented mutated proportion