Fig. 5.

The C₃ fullerene isomer protects cultured dopaminergic neurons from 6-OHDA-induced toxicity but only partially attenuates MPP⁺-mediated cell death.A, Representative fluorescent micrographs of TH-positive neurons treated with either 15 μm 6-OHDA or 1 μm MPP⁺ in the presence or absence of 75 μm C₃. B, Dose-dependent effects of C₃ on dopaminergic neurons treated with 15 μm 6-OHDA (•) or 1 μmMPP⁺ (○). The number of surviving dopaminergic neurons was determined 24 hr (6-OHDA) or 48 hr (MPP⁺) later by TH immunocytochemistry and normalized to the number of TH neurons in vehicle-treated plates. Data are mean ± SEM from determinations made from three cultures. *p < 0.01; **p < 0.001 compared with values from control plates (ANOVA with post hoc Student’st test).