Fig. 6.

Quantification of TH-positive cells in cultures of dissociated OB neurons alone. Pulses of NMDA increased the number of TH neuron in cultures of dissociated OB cells by 230% (p < 0.001). This induction was not affected by 1 μm TTX, suggesting that the direct effect of NMDA application does not require synaptic transmission. The addition of TTX in sham pulse controls slightly reduced the basal generation of TH neurons (p < 0.05), suggesting this level may in part be caused by spontaneous activity within the cultured OB neurons.