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. 1999 Feb 15;19(4):1307–1316. doi: 10.1523/JNEUROSCI.19-04-01307.1999

Fig. 2.

Fig. 2.

The mLin-2/CASK CKII domain directly binds to an X11α (region 373–436) peptide. A, GST fusion proteins described in Figure 1C were subjected to SDS-PAGE and transferred to nitrocellulose. Equivalent amounts of proteins were revealed with Ponceau red stain (left). The membrane was probed with soluble His-mLin-2/CASK (region 1–320) protein, and bound proteins were revealed with anti-T7 antibody, followed by HRP goat anti-mouse and chemiluminescence detection (right).B, The same procedure was performed to detect X11α in 293 cell lysate, except that soluble GST mLin-2/CASK (region 1–320) protein was used as primary reagent and anti-GST antibody–HRP protein A as secondary reagents. Increasing concentrations of soluble His-mLin-2/CASK (region 1–320) were mixed with a fixed concentration of soluble GST mLin-2/CASK (region 1–320) protein to compete for binding to X11α. A soluble His-X11α PDZ containing the two X11α PDZ domains was used as a negative control.