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. 1999 Feb 15;19(4):1307–1316. doi: 10.1523/JNEUROSCI.19-04-01307.1999

Fig. 8.

Fig. 8.

Colocalization of X11α and mLin-2/CASK in differentiated NT2 cells. A, Undifferentiated (Undif) or differentiated (Dif) NT2 cells treated with retinoic acid were lysed, and equal amounts of proteins were subjected to Western blot. The membrane was revealed with anti-X11 antibody (left). Cells were also stained with anti-X11 antibody and the nuclear stain 4-6-diamidino-2-phenylindole. White arrowindicates the restricted localization of X11α in a differentiated (Dif) neuron. The cell at the leftrepresents an undifferentiated (Undif) NT2 cell.B, Immunostaining of a differentiated NT2 cell with anti-X11 (left, Cy3-coupled secondary antibody) and anti-mLin-2/CASK (right, FITC-coupled secondary antibody). C, Immunostaining of a differentiated neuron with anti-X11 (left, FITC-coupled secondary antibody) and anti-giantin (right, Cy3-coupled secondary antibody) antibodies.