Ang II-induced nuclear translocation of PKB in WKY and SHR neurons. Neuronal cultures of WKY and SHR were incubated without or with 100 nm Ang II for the indicated time periods. Cytoplasmic and nuclear fractions were isolated and subjected to Western analysis as described in Materials and Methods.A, Cytoplasmic fractions; B, nuclear fractions. A, B, Top, Representative autoradiograms;bottom, quantitation of immunoreactive bands. Mean ± SE (n = 3). * Significantly different from control (p < 0.01).