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Effect of Ang II on PI3-kinase activity in hypothalami of adult WKY rats and SHRs. WKY rats and SHRs were cannulated and subjected to Ang II injection and isolation of hypothalamic blocks. Tissues were homogenized and used for the measurement of PI3-kinase activity as described in Materials and Methods. Top, Representative autoradiogram.Bottom, Quantitation of PIP spots from three animals. Data are mean ± SE (n = 3). * Significantly different from zero time (p < 0.01).
