Fig. 4.

Ang II-induced nuclear translocation of PI3-kinase in WKY and SHR neurons. Neuronal cultures of WKY rats and SHRs were incubated without or with 100 nm Ang II for indicated time periods. Cytoplasmic and nuclear fractions were isolated and subjected to Western analysis as described in Materials and Methods: (A) cytoplasmic fractions and (B) nuclear fractions. Whole-cell lysates from control and Ang II-treated cells were also run in separate experiments to determine levels of the enzyme. A, B,Top, Representative autoradiograms;bottom, quantitation of immunoreactive bands. Mean ± SE (n = 3). * Significantly different from control (p < 0.05).